Scientific Publications, Awards & Grants

Peer-reviewed publications:

  • Characterization and Crystallization of the Endoglucanase A from Clostridium Cellulolyticum in solution. Budayova M., Astier. J. P., Veesler S., Czjzek M., Belaich A., Boistelle R. (1999) J. Crystal Growth, 196, 297-304. DOI: 10.1016/S0022-0248(98)00873-2
  • Interactions in solution of a large oligomeric protein. Budayova M., Bonneté F., Tardieu A., Vachette P. (1999) J. Crystal Growth, 196, 210-219. DOI: 10.1016/S0022-0248(98)00844-6
  • Comparison of Solubility and Interactions of Aprotinin (BPTI) solutions in H2O and D2O. Budayova-Spano M., Lafont S., Astier J. P., Ebel C., Veesler S. (2000) J. Crystal Growth, 217, 311-319. DOI: 10.1016/S0022-0248(00)00495-4
  • Crystallization of a Recombinant Form of the Complete Sequence of Human gamma-Interferon: characterization by small-angle X-ray scattering, mass spectrometry and preliminary X-ray diffraction studies. Budayova-Spano M., Shepard W., Schoot B., Astier J. P., Veesler S. (2001) Acta Cryst., D57 (Pt 6), 900-905. DOI: 10.1107/S0907444901005820
  • Investigation of Aprotinin (BPTI) solutions during Nucleation. Budayova-Spano M., Bonneté F., Astier J. P., Veesler S. (2002) J. Crystal Growth, 235, 467-474. DOI:10.1016/S0022-0248(01)02001
  • The crystal structure of the zymogen catalytic domain of complement protease C1r reveals that a disruptive mechanical stress is required to trigger activation of the C1 complex. Budayova-Spano M., Lacroix M., Thielens N., Arlaud G.J., Fontecilla-Camps J.C., Gaboriaud C. (2002) EMBO J., 21 (3), 231-239. DOI: 10.1093/emboj/21.3.231
  • Structure, function and molecular genetics of human and murine C1r. Arlaud G. J., Gaboriaud C., Garnier G., Circolo A., Thielens N. M., Budayova–Spano M., Fontecilla -Camps, J. C., Volanakis J. E. (2002) Immunobiology, 205 (4-5), 365-382. DOI: 10.1078/0171-2985-00139
  • Structural biology of the C1 complex unveils the mechanisms of its activation and proteolytic activity. Arlaud G.J., Gaboriaud C., Thielens N.M., Budayova-Spano M., Rossi V. & Fontecilla-Camps J.C. (2002) Molecular Immunology, 39(7-8), 383-394. DOI:10.1016/S0161-5890(02)00143-8
  • Monomeric structures of the zymogen and active catalytic domain of complement protease C1r: further insights into the C1 activation mechanism. Budayova-Spano M., Grabarse W., Thielens N.M., Hillen H., Lacroix M., Schmidt M., Fontecilla-Camps J.C., Arlaud G.J. & Gaboriaud C. (2002) Structure, 10, 1509-1519. DOI: 10.1016/S0969-2126(02)00881-X
  • Production and X-ray crystallographic analysis of fully deuterated human carbonic anhydrase II. Budayova-Spano M., Fisher S. Z., Dauvergne M.-T., Agbandje-McKenna M., Silverman D. N., Myles D. A. A., McKenna R. (2006) Acta Cryst., F 62, 4-9. DOI:10.1107/S1744309105038248
  • Expression, purification, crystallization and preliminary X-ray analysis of a C-terminal fragment of the Epstein-Barr virus ZEBRA protein. Morand P., Budayova-Spano M., Perrissin M., Muller C., Petosa C. (2006) Acta Cryst., F 62, 210-214. DOI: 10.1107/S1744309106002971
  • A preliminary neutron diffraction study of Rasburicase, recombinant urate oxidase enzyme, complexed with 8-azaxanthin. Budayova-Spano M., Bonneté F., Ferté N., El Hajji M., Meilleur F., Blakeley M., Castro B. (2006). Acta Cryst., F 62, 306-309. DOI:10.1107/S1744309106006439
  • A methodology and an instrument for the temperature-controlled optimization of crystal growth. Budayova-Spano M., Dauvergne F., Audifren M., Bactivelane T., Cusack S. (2007). Acta Cryst., D63, 339-347. DOI: 10.1107/S0907444906054230
  • Atomic Crystal and Molecular Dynamics Simulation Structures of Human Carbonic Anhydrase II: Insights into the Proton Transfer Mechanism. Fisher S. Z., Maupin C. M., Budayova-Spano M., Govindasamy L. Tu C., Agbandje-McKenna M., Silverman D. N., Voth G. A., and McKenna R. (2007). Biochemistry, 46, 2930-2937. DOI: 10.1021/bi062066y
  • Large crystal growth by thermal control allows combined X-ray and neutron crystallographic studies to elucidate the protonation states in Aspergillus flavus urate oxidase. Oksanen E., Blakeley M. P., Bonneté F., Dauvergne M-T., Dauvergne F., and Budayova-Spano M. (2009). Journal of the Royal Society Interface, 6 (Suppl. 5), S565 – S696. DOI:10.1098/rsif.2009.0162.focus
  • Design of a novel Peltier-based cooling device and its use in neutron diffraction data collection of perdeuterated yeast pyrophosphatase. Oksanen E., Dauvergne F., Goldman A., and Budayova-Spano M. (2010). Journal of Applied Crystallography, 43, 1113-1120. http://dx.doi.org/10.1107/S0021889810027111
  • The neutron structure of urate oxidase resolves a long-standing mechanistic conundrum and reveals unexpected changes in protonation. Oksanen E., Blakeley M. P., El-Hajji M., Ryde U., and Budayova-Spano M. (2014). PLoS ONE, 9(1), e86651. DOI:10.1371/journal.pone.0086651
  • A crystallization apparatus for temperature-controlled flow-cell dialysis with real-time visualization. Junius N., Oksanen E., Terrien M., Berzin M., Ferrer J.L. and Budayova-Spano M. (2016). Journal of Applied Crystallography, 49, 806-813. http://dx.doi.org/10.1107/S1600576716004635

 

Publication of scientific popularization:

“Cristalliser les protéines pour les observer en trois dimensions”: Monika Budayova-Spano, Visions Croisées / Journal des universités de Grenoble, Magazine n° 4 / Spring 2009, 10-11.

 

Patents:

  • Patent FR10/57354 UJF 2010 (EP 117730945, US13821053, JP2013528746) entitled «Device and method for crystallizing inorganic or organic substances», published: 15/9/2011.

Author: Monika Spano. A license for this patent and the related know-how is granted to NatX-ray SAS (Saint Martin d’Hères, France: http://www.natx-ray.com/products/catalogue_subsys_PRT008.html).

  • Patent FR2016/053185: «Puce microfluidique pour la cristallisation de molécules, procédé de préparation, dispositif la comprenant et procédé de cristallisation de molécules.»

Authors: Budayova-Spano M., Junius N., Salmon J. B.

 

Prizes, awards & grants:

  • Young Scientist Award, 11th International Conference on the Crystallization of Biological Macromolecules, Quebec, Canada, August 2006.

Laureate of:

  • CRISTECH funding grant, period 2010/2011, from MRCT/CNRS: “An integrated platform for the OPTImization of CRYStal growth (OPTICRYS)”.
  • DEFI Instrumentation at the limits grant, period 2014-2015, from MI/CNRS: “Crystallization with an AuTomated microfluidic pipeLINE: from crystal screening to crystal mounting (CATLINE)”.
  • Valo-GRAL Labex grant, period 2015, from Grenoble Alliance for Integrated Structural & Cell biology: “An integrated platform for the OPTImization of CRYStal growth (OPTICRYS)”.
  • Marie Sklodowska-Curie Actions H2020-MSCA-ITN-2016 grant, period 2017-2021, from EU: “RAtionalising Membrane Protein crystallisation (RAMP)”.

 

Invited seminars/talks/lectures for national & international conferences, workshops & schools:

~ 30 invitations in the period: 2010-now